Optimization of fermentation conditions for the expression of interleukin-3 in fusion with pelb in E. coli
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Optimization of Cloning Conditions for high-level Production of Recombinant Mouse Interleukin-2 in Escherichia coli
Backgrounds and objectives: Interleukin 2 (IL-2) secreted by activated CD4+ T cells has been known as a major mediator in both adaptive and native immune system due to a board range of effects on different cells in the immunity system (1-6). Methods: cDNA synthesis was performed using gene- specific primers designed by Gene Runner software after RNA extraction of mouse splenocytes. PCR pro...
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ژورنال
عنوان ژورنال: TAP CHI SINH HOC
سال: 2016
ISSN: 0866-7160,0866-7160
DOI: 10.15625/0866-7160/v38n2.7126